The interaction between nucleophosmin/NPM1 and the large …
网页2021年1月1日 · RNAs were purified and analyzed by agarose gel electrophoresis and proteins were detected by western blotting with anti-NPM1 antibody (top and bottom panels, respectively). Under a low Mg 2+ concentration, the purified ribosomes were fractionated by a 15%–40% sucrose density gradient.
Molecular study of Nucleophosmin 1 (NPM1) gene in acute …
网页GEL Electrophoresis. Ten µl of the PCR product was electrophoresed on 2.5% agarose gel (Bioneer), using 100 bp DNA ladder. The one MARK 100 with novel green was optimized for direct loading on to unstained agarose gels.
Rapid and highly specific screening for NPM1 mutations in
网页2012年11月17日 · In this report, we show that FAST PCR and subsequent gel electrophoresis is a specific and robust tool for the screening of NPM1 mutations and can be performed in less than 2 h. PCR products are run on a ready-to-use 2 % agarose gel that clearly separates the mutant from the wild-type band.
Establishment of a Multiplex PCR-Based Procedure for Detection …
网页Multiplex PCR products and digestion products were run on 3% Ultraphor™ agarose gel at 400 volts for 45 minutes at 4°C. Electrophoresis gel was stained with 0.5 µg/ml ethidium bromide solution and then visualized by GelDoc system (Bio-Rad). Results. Establishment of multiplex PCR-based procedure for detection of three mutations.
网页agarose gel electrophoresis. We have recently shown that alterations in the NPM1 gene represent the most common genetic lesion currently detectable
Simultaneous detection of NPM1 and FLT3-ITD mutations by
网页2005年6月23日 · The approach most commonly used for large-scale diagnostic screening of Flt3 ITD consists of RT-PCR followed by agarose gel electrophoresis. We have recently shown that alterations in the...
Agarose Gel Electrophoresis for the Separation of DNA Fragments
网页2012年4月20日 · Agarose gel electrophoresis has proven to be an efficient and effective way of separating nucleic acids. Agarose's high gel strength allows for the handling of low percentage gels for the separation of large DNA fragments. Molecular sieving is determined by the size of pores generated by the bundles of agarose 7 …
Molecular Genetic Tests for FLT3, NPM1, and CEBPA in Acute …
网页2013年1月1日 · Molecular tests for mutations in FLT3, NPM1, and CEBPA genes can be performed using genomic polymerase chain reaction (PCR), followed by capillary gel electrophoresis (fragment sizing) for FLT3-ITD mutations and NPM1 exon 12 insertions; restriction enzyme digestion with EcoRV for identification of the FLT3 D835 mutation; and PCR followed by ...
Figure 1. Agarose gel electrophoresis of NPM1 gene (exon 12): …
网页Agarose gel electrophoresis of NPM1 gene (exon 12): Lane 1 = Normal control sample. Lanes 2, 5, 6, 7, 8, 9, 10, 11, 12, 13, and 15 represent wild-type NPM1 gene. Lanes 3, 4, and 14...
Addgene: Protocol - How to Run an Agarose Gel
网页Gel electrophoresis is the standard lab procedure for separating DNA by size (e.g., length in base pairs) for visualization and purification. Electrophoresis uses an electrical field to move the negatively charged DNA through an agarose gel …
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