FlowJo [APOPTOSIS ANALYSIS] - YouTube
网页Are you new to data analysis in FlowJo or looking for a starting point? This video will give you a quick overview on analyzing your apoptosis (Annexin V / PI...
易选生物丨FIow Jo 分析细胞凋亡，教程详解！ - 知乎专栏
网页2024年1月24日 · Annexin V和PI双染法是流式检测细胞凋亡的经典方法，它是基于凋亡的早期细胞膜上的磷脂酰丝氨酸（phosphotidylserine, PS）从细胞膜的内侧翻转到细胞膜的表面这一原理来实现的。. Annexin Ⅴ（膜联蛋白 V）是一种分子量为35-36 kDa的Ca2+依赖性磷脂结合蛋白，能与PS高 ...
Determining the contents and cell origins of apoptotic bodies by …
网页2017年10月31日 · Flow cytometry analysis showing the six-stage electronic gating strategy used to identify ApoBDs with different amount of mitochondria from a sample containing a mixture of viable cells, apoptotic...
7 Tips For Measuring And Reporting Apoptosis By Flow Cytometry
网页FlowJo is a powerful tool for performing and analyzing flow cytometry experiments, if you know how to use it to the fullest. This includes understanding embedding and using keywords, the FlowJo compensation wizard, spillover spreading matrix, FlowJo and R, and creating tables in FlowJo.
Univariate Hints - FlowJo Documentation
网页If apoptosis is an important aspect of your analysis, use a marker for apoptosis such as Annexin V. It is easy to show that many of the sub-G1 cells are debris and not apoptotic, and that many of the apparent G0/G1 cells are in early apoptosis, and thus using DNA content alone as a judge of apoptosis gives inaccurate results.
How to Use Flow Cytometry to Measure Apoptosis: Part One
网页Flow cytometry is a powerful tool for measuring apoptosis. Unlike techniques like western blotting, it provides an efficient way to characterize lots of cells individually rather than studying a mixed population.
Monitoring the progression of cell death and the disassembly of …
网页2016年3月3日 · To study the progression of cell death in greater detail, in particular how apoptotic cells undergo fragmentation to generate membrane-bound vesicles known as apoptotic bodies, we established a...
Flow cytometry-based apoptosis detection - PMC - National …
网页Apoptosis of human follicular lymphoma cells was induced with dexamethasone (Dex). Ethanol fixed and propidium iodide (PI) stained cells were analyzed on a flow cytometer. Red fluorescence of PI was collected using linear amplification scale.
网页FlowJo is an integrated environment for viewing and analysing flow cytometric data, presented in the form of a Workspace. The Workspace contains a list of all of the data samples that you load, the gates, statistics and other analyses that you apply, and the table and graphical layout that you design.
Measurement and Characterization of Apoptosis by Flow Cytometry
网页2016年7月1日 · Flow cytometry has been the method of choice for analyzing apoptosis in suspension cells for more than 25 years. Numerous assays have been devised to measure both the earliest and latest steps in the apoptotic process, from the earliest signal-transduction events to the late morphological changes in cell shape and granularity, proteolysis, and ...