GelPilot DNA Molecular Weight Markers - QIAGEN
网页GelPilot markers are provided ready to load in gel loading buffer. The unique buffer contains 3 tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time (see figure " GelPilot Loading Buffer ").
Customise the MW marker List - SnapGene Support
网页SnapGene has hundreds of commercial and common molecular weight markers (MW markers) that you can use with your agarose gel simulations. Not all markers are listed by default. Follow the instructions in this lesson to add your favorite markers to the default list.
网页Use the same DNA loading dye (supplied with the DNA ladder/marker) for both the sample DNA and the ladder/marker DNA. ual volumes of the sample DNA and the ladder/marke. Avoid high salt concentrations in the DNA samples as …
DNA Markers and Ladders - NEB
网页Ladders are available for agarose gel electrophoresis and pulsed-field gel electrophoresis. Need help selecting a DNA marker or ladder? Try our DNA Markers & Ladders Selection Tool. Convenient DNA ladder formats. Conventional. (with no loading dye) Available in a wide variety of size ranges. Most economical format. Quick-Load ® Purple format.
Module 1.2: Agarose Gel Electrophoresis - MIT OpenCourseWare
网页Ethidium Bromide is a fluorescent dye that is commonly added to agarose gels. This dye intercalates between the bases of DNA, allowing DNA fragments to be located in the gel under UV light and photographed. The intensity of the band reflects the concentration of molecules that size, although there are upper and lower limits to the sensitivity ...
Agarose Gel Electrophoresis for the Separation of DNA Fragments
网页2012年4月20日 · By following this protocol, students should be able to: 1. Understand the mechanism by which DNA fragments are separated within a gel matrix 2. Understand how conformation of the DNA molecule will determine its mobility through a gel matrix 3. Identify an agarose solution of appropriate concentration for their needs 4.
DNA molecular weight markers - takarabio.com
网页Our selection of DNA ladders and DNA molecular weight markers—with fragment sizes ranging from 20 bp to 2.5 kb—are suitable as size standards for various applications, such as nucleic acid size and quantity determination during agarose gel electrophoresis.
Choose a MW Marker - SnapGene Support
网页2 years ago. How do I choose a MW marker for an agarose gel simulation? Select the MW Marker Lane. To select the MW Marker lane, click "1" above the gel. Choose the Ladder. Expand the MW Marker menu, then choose the desired ladder. If we have overlooked a MW marker that you use, please contact us and we will add it to the list. View the Ladder.
TriDye™ Ultra Low Range DNA Ladder - NEB
网页TriDye™ Ultra Low Range DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing three tracking dyes which serve as visual aids to monitor the progress of migration during native agarose and polyacrylamide gel electrophoresis.
TriDye™ 1 kb Plus DNA Ladder - NEB
网页TriDye™ 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands ...
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