Systematic design of microscope objectives. Part I: System …
网页The microscope objective lens is the most typical high numerical aperture (NA) system, which provides a high-contrast image with diffraction-limited resolution for various microscopy applications. The development of microscope objective has a long history over hundreds of years.
Understanding Microscopes and Objectives - Edmund Optics
网页Most microscope objective specifications are listed on the body of the objective itself: the objective design/standard, magnification, numerical aperture, working distance, lens to image distance, and cover slip thickness correction.
网页Characteristics of microscope objectives • Historically, most microscope objectives were designed to operate at finite conjugates – “Infinity-corrected” becoming more prevalent • Specified by NA in object space – object marginal angles tend to be large (fast) • Must resolve fine detail or structure of SUT
Systematic design of microscope objectives. Part II: Lens modules …
网页To demonstrate the lens modules more clearly, in this paper, we focus on the microscope objectives from Zones 1–4, which generally consist of two or three groups with distinctive functionalities. The detailed lens modules utilised in the front, middle and rear group are discussed in Sections 4–6, respectively.
Microscope Objectives - Introduction | Olympus LS - Olympus …
网页Objectives are designed to image specimens either with air or a medium of higher refractive index between the front lens and the specimen. The field of view is often quite limited, and the front lens element of the objective is placed close to the specimen with which it must lie in optical contact.
网页The modern microscope objective is the most sophisticated optical component in light microscopes, providing high contrast images with diffraction-limited resolution. The design of microscope objectives has been developed for over a hundred of years. However, after 1970s, the review of advanced application-oriented systems is limited, and the design
网页We are summarising the conventional design principles of the microscope objective design, which are valid in more than 80% of corresponding systems. They can be classified into three categories: optical power distribution, material selection and lens structures. The combination of. © The Authors, published by EDP Sciences.
Systematic design of microscope objectives. Part III: …
网页In this paper, the lens modules used in the Zones 1–4 microscope objectives, which have been summarised in Part II, are utilised to create new structures. Both the modification of available systems and the synthesis of new system …
Rethinking microscope objectives - Nature Methods
网页2023年5月11日 · A microscope’s performance is often constrained by the characteristics of the objective lens, which limit features such as spatial resolution, magnification, field of view and working...
Systematic design of microscope objectives. Part II: Lens modules …
网页2019年6月7日 · A new expansion-assisted selective plane illumination microscope (ExA-SPIM) with aberration-free 1×1×3 μm optical resolution over a large field of view and working distance is presented, which allows imaging centimeter-scale samples with 250×250×750 nm optical resolution (4× expansion), including …
跳至内容