Rapid non-destructive method to phenotype stomatal traits
网页2023年3月31日 · A commonly used method to investigate stomatal traits is a nail polish method (NP method) [10,11,12,13,14,15]. It consists of applying nail polish on the leaf surface to get a leaf imprint, waiting for the polish to dry, carefully peeling off the polish, examining the leaf imprint under a light microscope, taking and analysing images to ...
How To View Stomata Under The Microscope - Science Lessons …
网页Slowly peel the tape off of the leaf. The nail polish should now be stuck to the tape. Place the tape directly onto the microscope slide and place it under the microscope. Depending on the leaf type, students will generally need to be on at least 100x to see them clearly.
Using Clear Nail Polish to Make - Springer
网页2017年2月21日 · Suitable clear nail polish can instantly fix the status of stomatal guard cells and provides clear, stable, and almost permanent slides of epidermal impressions for measurement of stomatal aperture size.
Using Clear Nail Polish to Make Arabidopsis Epidermal ... - PubMed
网页Here, we described a detailed protocol of using clear nail polish to make Arabidopsis epidermal impressions for investigate the change of stomatal aperture size in plant immune response. The application of this approach can instantly fix the status of stomatal guard cells, and provides clear, stable, and almost permanent slides of epidermal ...
Novel perspectives on stomatal impressions: Rapid and non
网页2020年9月3日 · A powerful alternative to the methods described above is the preparation of replicas or impressions of leaves by using nail polish, rubber, glue, or silicone and the investigation with light microscopy [15–19] and SEM [20–23], which allows for in vivo physiological inference, for example of stomatal conductance.
StomataScorer: a portable and high‐throughput leaf stomata trait …
网页The most traditional method of stomata imaging is blotting, belonging to destructive, which the stomatal morphology is fixed with a fixator for observation with a microscope (Gitz and Baker, 2009; Iodibia et al., 2017). In recent years, some works have been done to improve these traditional methods.
From leaf to label: A robust automated workflow for stomata …
网页2020年8月19日 · The nail polish method was successfully applied in 78% of the species sampled. Generating impressions failed in 16% of the species due to hairy or velvety leaf surfaces. In 7% of the species for which we managed to get leaf prints, we were unable to detect the stomata visually.
The relationships between photosynthesis and stomatal traits …
网页2020年9月1日 · The nail-polish imprint method was used to examine stomatal traits (Zhao et al., 2016). Clear nail polish was applied onto the middle of the abaxial leaf surface, allowed to dry, then pulled off the leaf by using sellotape and mounted onto a glass slide.
Microscopy image recognition method of stomatal open and
网页2023年10月13日 · Stomata are epidermal pores in leaves, stems, and other parts of plants and are structures unique to the plant epidermis. Their movement is generally regulated by the opening and closing of guard cells, and the behavior of stomata is of great significance in the physiological study of plants.
Rapid non-destructive method to phenotype stomatal traits
网页2022年6月30日 · The method commonly used to investigate stomatal traits is the nail polish method. It consists of applying nail polish on the leaf surface to make an imprint that includes stomata, examining the leaf imprints under a light microscope, and analyse images manually to determine the stomata number and size …