Pyrosequencing - Wikipedia
网页Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released.
Pyrosequencing: Principle, Steps, Reactions, Types, Uses
网页2024年8月4日 · Pyrosequencing is a sequencing method that determines the sequence of nucleotides by detecting the release of pyrophosphate (PP i) during nucleotide incorporation and monitors DNA synthesis in real-time. Pyrosequencing relies on light detection which occurs due to a chain reaction initiated by the release of pyrophosphate.
Pyrosequencing Technology and Platform Overview - QIAGEN
网页Pyrosequencing is a sequence-based detection technology that enables rapid and accurate quantification of sequence variation. Streamlined protocols, analysis flexibility, and elegant output make Pyrosequencing technology a highly adaptable tool for exploratory and testing work in a broad range of disciplines.
焦磷酸测序 - 百度百科
网页焦磷酸测序技术 (pyrosequencing)是一种新型的酶联级联测序技术，焦磷酸测序法适于对已知的短序列的测序分析，其可重复性和精确性能与SangerDNA测序法相媲美，而速度却大大的提高。. 焦磷酸测序技术产品具备同时对大量样品进行测序分析的能力，为大通量、低 ...
Pyrosequencing - an overview | ScienceDirect Topics
网页Pyrosequencing is a nongel-based DNA sequencing technique that detects inorganic pyrophosphate released during DNA synthesis, allowing for faster, cheaper, and semiquantitative analysis of nucleotide incorporation compared to traditional methods like Sanger sequencing.
Pyrosequencing: powerful and quantitative sequencing technology
网页2023年7月15日 · Pyrosequencing is a sequencing-by-synthesis method for DNA analysis that has emerged as a platform not only for de novo sequencing applications, but also for quantitative analysis of genomic methylation, single-nucleotide polymorphisms, and allele quantification.
Pyrosequencing » Genetic Resources Core Facility, Johns Hopkins ...
网页Pyrosequencing is a method of sequencing that relies on light emitted in an enzymatic reaction set in motion by the release of pyrophosphate when a base is added during the sequencing process. The results are quantitative, making the method ideal for sequencing samples of known sequence that may have mutations in …
Fundamentals of pyrosequencing - PubMed
网页2013年9月3日 · We demonstrate how mutant and wild-type DNA sequences result in different pyrograms. Using pyrograms of established mutations in tumors, we explain how to analyze the pyrogram peaks generated by different dispensation sequences. Further, we demonstrate some limitations of pyrosequencing, including h ….
Pyrosequencing: History, biochemistry and future - ScienceDirect
网页2006年1月1日 · Pyrosequencing is a DNA sequencing technology based on real-time detection of DNA synthesis monitored by bioluminescence. Four enzymes are exploited by the technology and a fifth protein, SSB, can be included to enhance the quality of the obtained sequences and thereby prolong the read length.
Pyrosequencing - SpringerLink
网页Pyrosequencing, a bioluminometric DNA sequencing technique based on sequencing by synthesis, is emerging as a widely applicable tool for detailed characterization of nucleic acids (1–3). This technique relies on the real-time detection of inorganic pyrophosphate (PPi) released on successful incorporation of nucleotides during DNA synthesis.
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