网页While the methods described for direct flank-to-flank tumor injection for serial passaging can be scaled up to inject large numbers of animals with flank tumors, we have found that injection of flank tumors using short-term cultured cells provides more reproducible and uniform tumor growth than does direct tumor passaging (unpublished data), whi...
Development and characterization of patient-derived xenografts …
网页2021年1月28日 · Here, we report on the development and characterization of a cohort of NSCLC brain metastasis PDX models, including both flank and orthotopic intracranial xenografts as well as two NSCLC brain...
Characterization of human cancer xenografts in humanized mice
网页2020年3月26日 · Humanized models represent a powerful tool to assess preclinical activity of immuno-oncology (IO) agents. In this report, we characterized several commonly used xenograft models spanning a range of tumor types for immunophenotypic profile, expression of PD-L1 and PD-1 on tumor and immune …
The initial engraftment of tumor cells is critical for the future ...
网页2020年6月5日 · Xenograft mouse tumor models are used to study mechanisms of tumor growth and metastasis formation and to investigate the efficacy of different therapeutic interventions. After injection the engrafted cells form a local tumor nodule.
Investigation of tumor-tumor interactions in a double human …
网页2018年4月4日 · In this model, a first tumor xenograft was induced subcutaneously (s.c.) by injection of the HPV-16 cervical carcinoma SiHa cell line into one anatomical site (right flank) and later on, animals were challenged with tumor cells injected subcutaneously into a distant anatomical site (contralateral flank).
A Novel Cell Line Based Orthotopic Xenograft Mouse Model That ...
网页2017年12月19日 · To generate liver tumors, we injected two million HepG2 or Huh-6 human HB cell lines into either the right median lobe with a right flank incision (Fig. 1a) or the left lateral lobe with a...
Understanding Cell Lines, Patient-Derived Xenograft and …
网页2022年2月9日 · Lymphocytes isolated from skin of IL-15 Tg CTCL mice were administrated in SCID mice. Approximately, 2 × 10 6 cells inoculated subcutaneously into the right flank of each SCID mouse led to CTCL development.
Cell Press: STAR Protocols
网页2021年4月8日 · To investigate whether a similar mechanism is applicable in human tumors as well, we used this protocol for the selection of IL-34-neutralizing antibody and transplanting human tumor tissue expressing both IL-34 and PD-L1 as a patient-derived xenograft in immunologically humanized mice.
Xenograft and organoid model systems in cancer research
网页2019年7月8日 · Whereas the first patient‐derived tumour xenograft (PDTX) models were successfully established during the fifties (Toolan, 1953), patient‐derived tumour organoid (PDTO) models have been established only during the last decade (Sato et al, 2011) (Fig 1).
Tracking Tumor Colonization in Xenograft Mouse Models Using Accelerator ...
网页2018年10月9日 · Here we introduce an Accelerator Mass Spectrometry (AMS)-based high precision method for quantifying the number of cancer cells that initiate metastatic tumors, in xenograft mice.