Pyrosequencing - Wikipedia
网页Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase.
Pyrosequencing: Principle, Steps, Reactions, Types, Uses
网页2024年8月4日 · Pyrosequencing is a sequencing method that determines the sequence of nucleotides by detecting the release of pyrophosphate (PPi) during nucleotide incorporation and monitors DNA synthesis in real-time.
Pyrosequencing - an overview | ScienceDirect Topics
网页Pyrosequencing is a nongel-based DNA sequencing technique that detects inorganic pyrophosphate released during DNA synthesis, allowing for faster, cheaper, and semiquantitative analysis of nucleotide incorporation compared to traditional methods like Sanger sequencing.
Pyrosequencing Technology and Platform Overview - QIAGEN
网页Characterization of complex DNA modifications underlying gene expression patterns requires quantifiable sequence data. Pyrosequencing is a sequence-based detection technology that enables rapid and accurate quantification of sequence variation.
Pyrosequencing: powerful and quantitative sequencing technology
网页2023年7月15日 · Pyrosequencing is a sequencing-by-synthesis method for DNA analysis that has emerged as a platform not only for de novo sequencing applications, but also for quantitative analysis of genomic methylation, single-nucleotide polymorphisms, and allele quantification.
Fundamentals of Pyrosequencing | Archives of Pathology
网页2013年9月1日 · Pyrosequencing is less complex, involves fewer steps, and has a superior limit of detection compared with Sanger sequencing. The fundamental basis of pyrosequencing is that pyrophosphate is released when a deoxyribonucleotide triphosphate is added to the end of a nascent strand of DNA.
Pyrosequencing » Genetic Resources Core Facility, Johns Hopkins ...
网页What is pyrosequencing? Pyrosequencing is a method of sequencing that relies on light emitted in an enzymatic reaction set in motion by the release of pyrophosphate when a base is added during the sequencing process.
Pyrosequencing - an overview | ScienceDirect Topics
网页Pyrosequencing is a replication-based sequencing method in which addition of the correct nucleotide to immobilized template DNA is signaled by a photometrically detectable reaction. From: Epigenetics in Human Disease , 2012
Pyrosequencing - an overview | ScienceDirect Topics
网页Pyrosequencing was described in 1985 [1] and is currently used for quantitative analysis of nucleic acids. It is a method where the sequencing takes place during the deoxyribonucleic acid (DNA) synthesis, using the so-called “sequencing-by-synthesis” principle.
Fundamentals of pyrosequencing - PubMed
网页2013年9月3日 · Pyrosequencing is less complex, involves fewer steps, and has a superior limit of detection compared with Sanger sequencing. The fundamental basis of pyrosequencing is that pyrophosphate is released when a deoxyribonucleotide triphosphate is added to the end of a nascent strand of DNA.
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