3| Separate the fragments of DNA by electrophoresis through an agarose gel (for example, a 0.7% gel cast in 0.5× TBE or 1× TAE buffer). Maintain a low voltage through the gel (≤1 V/cm).

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In this form of pulsed-field gel ... electrophoresis (CHEF) 2 is recommended. This protocol describes the resolution of genomic DNA by TAFE, followed by blotting and hybridization. 1 | Cast a 1% ...

Before electrophoresis begins, a user prepares and cools a liquid gel, pours it into a tray, and fits it with a toothed comb to create separations for each individual sample. A buffer fluid containing ...