基因敲入所面临的主要难题是修复模板的制备和如何与Cas9-sgRNA核糖核蛋白复合物共导入至细胞。虽然单链DNA（ssDNA）修复模板展示出了优于双链DNA（dsDNA）模板的众多优势，但是其制备难度和制备费用都有很大的挑战性，因而限制了长单链DNA（long ssDNA）模板的 ...

基因敲入（knockin）实验所面临的主要挑战之一就是供体模板的制备。 双链DNA(dsDNA)和单链DNA(ssDNA)都可以作为HDR修复机制的供体模板。不过，质粒或者PCR片段这样的dsDNA供体模板存在随机整合到基因组上的风险，在准确的基因编辑上的应用是非常有限的。而ssDNA ...

The E-Gel ® electrophoresis system is ideal for routine analysis of PCR products, restriction digests, and plasmid preparations. No gels to pour, no buffer to make, no staining/destaining ...

Protocol is a format for step-by-step descriptions of procedures that users can take to the lab and immediately apply in their own research. All of our protocols have been proven to work already ...

The Kyoto Protocol, the first international treaty to set legally binding targets to cut greenhouse gas emissions, was adoped 25 years ago, on 11 December 1997, in Kyoto, Japan. The agreement ...

A sequence of operations that ensure protection of data. Used with a communications protocol, it provides secure delivery of data between two parties. The term generally refers to a suite of ...

If you prioritize a cool and comfortable sleep experience, a gel mattress can make a world of difference. We tested several options and here are our top picks. McKenzie, a Certified Sleep Science ...

One more restrictive diet plan you may hear about is the autoimmune protocol (AIP) diet. It’s based on the idea that certain foods inflame your gut and that eliminating them may ease autoimmune ...

Alice Morgan / Investopedia File transfer protocol (FTP) is a standard communication protocol used to transfer computer files from one location to another via the Internet. A massive amount of ...

Why was Blue Protocol canceled? Unveiled at The Game Awards 2022, weeby free-to-play game Blue Protocol came hot on the heels of the successes of Genshin Impact and Honkai Star Rail, introduced an ...

Those who live and work there sometimes appear less bothered about protocol than critics watching from outside. The recent obituaries of Lord Fellowes, who served as private secretary to Elizabeth ...