1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

It uses the fact that denatured nucleic acids will bind to nitrocellulose or nylon membranes. DNA fragments are separated by size on a gel, then transferred to the membrane by capillary transfer or ...

Scientists who study protein-DNA interactions frequently rely upon gel shift assays or electrophoretic mobility shift assays (EMSAs), which are based on relative differences in electrophoretic ...