As p-nitrophenol is a skin irritant, you should wear gloves throughout these assays. 2. Prepare a sub-stock by diluting 100 µl of original ... 5.5 mM). 5. Now you have to determine enzyme activities ...

They exhibit remarkable sensitivity, wide dynamic range and robust performance that compares advantageously with conventional enzyme-linked immunosorbent assay (ELISA). ELISA is the most widely ...

The Transcreener ADP 2 Assay uses a highly selective antibody and far-red fluorescent tracer to directly detect ADP produced by any ADP-producing enzyme (i.e. kinases, ATPases, helicases ...

Figure 1: All assays based on HTRF KinEASE involve two steps: the enzymatic step and the detection step with HTRF reagents. SEB titration: for kinases, a Supplement Enzymatic Buffer (SEB ...

The unique direct detection format simplifies the protocol (no coupling enzyme needed) and minimizes compound interference. Because the assay is based on direct SAH detection, it is well-suited ...

One major challenge in assay development and lyophilization is incompatibility with conventional enzyme formats. It is imperative that enzymes used do not contain glycerol in their buffers, as this ...

Researchers from the Chinese Academy of Sciences and Nanjing University of Chinese Medicine have engineered a more efficient ...

In a recent study published in Engineering, researchers have made significant strides in the enzymatic depolymerization of polyethylene terephthalate ...