Simple stains with fluorochrome dyes—acridine orange, for example—are inadequate for diagnostic use. Two methods which have hitherto been used for the demonstration of polysaccharides proved ...

However, choosing optimal antibody-fluorochrome combinations is a complex process. Here we provide some simple guidelines for the selection of reagent panels for multicolor flow cytometry.

Spectral flow cytometry collects the full emission spectrum of a fluorochrome, enabling multicolor panels with more parameters than conventional flow cytometry. As scientists discover the subtle ...