1. Prepare a 40 ml 1 % agarose gel in 0.5X TBE, containing 0.5 µg/ml ethidium bromide. Each gel will be shared by 2 groups on the same bench. 3. Each group will load PCR and control samples on one ...

Run the gel in the same buffer solution as used for preparing the gel. 2 | Prepare agarose plugs containing the DNA of interest and carry out digestion with restriction enzymes. Prepare and embed ...

Before capillary transfer, several steps must be taken to prepare the DNA for transfer to the membrane. The gel should first be examined under UV light to confirm ... (The needle should be pushed ...

When you want really tasty, buttery pastry, it is best to make your own from scratch. This rough puff is an easier version of the full puff pastry, but no less delicious. Make double and freeze ...

If the mixture is too stiff, add a little more milk. Scrape down the sides of the bowl to make sure all the icing sugar is incorporated. Continue to whisk until light and creamy. You can colour ...

Don't we all long to know the secret to how to make a gel manicure last longer? Luckily, social media nail influencers have ...

Using the right brow gel is just as important as applying it correctly. Here, freelance beauty editor Morgan Fargo demonstrates how its done. Eyebrow trends come and go. From thick and fluffy to ...

Assemble five key points to make during your interview. Research the company as well as its branding and online presence. Practice your answers to common interview questions. Arrive early and ...

Gel ice packs are just as easy to make and so much more comfortable to use than those hard, clunky old ice cube-filled plastic bags. They’re also more durable, reusable and flexible, so they can ...

Today I want to discuss the steps you can take right now to prepare for one. If we enter a recession, you’ll be ready. If we don’t, your finances will be better off. It’s a win-win scenario.

Make simple repairs - One of the biggest turn-offs for buyers is wear and tear such as chipped tiles, cracked windows, scuffed paintwork, stained carpets, and other obvious signs of damage.

Analyze the size and quality of the products by electrophoresis through an agarose gel. Select several colonies, grow each in 5 ml of 2YT medium with the appropriate antibiotic and prepare plasmid ...