By Vijay Kumar Malesu New genetic tools reveal the dual roles of senescent cells in liver disease—macrophages drive fibrosis, ...
Also, for ES cell assays it is important to include a 'mock' transfection control containing Lipofectamine 2000 alone, as this reagent may not be biologically inert. For example, we consistently ...
After plating 4000 cells/well in 96-well dishes, cells were transfected with 15 ng plasmid/well using lipofectamine and collected 72 h post-transfection, using the conditions described in the next ...
This useful study by Gao et al identifies Hspa2 as a heterogeneous transcript in the early embryo and proposes a plausible mechanism showing interactions with Carm1. The authors propose that ...
In this important study, the authors used a zebrafish model and scRNAseq analysis to show that a subset of keratinocytes within melanoma microenvironment highly up-regulate Twist and undergo ...