Cells are homogenized in guanidinium thiocyanate and the RNA is purified from the lysate by extraction with phenol-chloroform at reduced pH 1. This method allows many samples to be processed ...

Set up two reactions for digestion of genomic DNA: Incubate the two reactions at 37 °C for 3 h. Extract the reactions with phenol/chloroform (1:1, v/v), recover the DNA by precipitation with ...