Phos-tag™ SDS-PAGE是使用了Phos-tag™ Acrylamide或SuperSep的SDS-PAGE。可通过条带的迁移率差异，指示磷酸化蛋白的磷酸化水平。 1.SDS-PAGE胶中的Phos-tag™ 捕获磷酸化蛋白。 2. 电泳中的磷酸化蛋白的泳动速度变慢。 3. 根据磷酸化水平出现在上移后的位置，分离出磷酸化 ...

Phos-tag™ Acrylamide SDS-PAGE可根据迁移率不同，区分磷酸化蛋白与非磷酸化蛋白。该方法只需在常规的SDS-PAGE胶中添加Phos-tag™ Acrylamide和MnCl 2 即可进行实验。 酪氨酸激酶Abl作用于带有GST标签的底物ABLTIDE，采用Phos-tag™ SDS-PAGE，可检测底物上酪氨酸的磷酸化反应。

Since its introduction in 1979, the traditional Western blot has remained a lengthy and laborious analytical process. But by taking an automated approach, researchers can reduce errors, enhance data ...

A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue ...

Assessing the relative change in protein expression is a valuable and versatile tool for a wide range of basic and applied research. Scientists routinely use quantitative western blots to investigate ...

Western blotting allows investigators to detect a specific protein in a complex mixture. Download this guide to discover tips on optimizing the gel and loading samples, antibody validation and ...

neuronal cell bodies, dendrites and some thick axonsin the central and peripheral nervous systems, but not thin axons;reflects extent of aberrant phosphorylation of neurofilaments in ...

Sci-Hub, using legally dubious methods, makes academic research free to researchers worldwide. It is a win for Western academia.

To understand and emulate Singapore’s success, other countries must try to understand the mind of its visionary leader.

Volleyball coaches association releases Week 2 WPIAL girls rankings The Western PA Volleyball Coaches Association’s WPIAL girls volleyball top-10 rankings for Week 2 were released Monday morning ...

Leading companies Phison and Western Digital (WD)... JCET and Western Digital (WD) agreed on an equity purchase agreement, under which the former will acquire most of the stake in the latter's ...