2). The ratio of plasmid DNA to transfection agent within polyplexes had a major impact on transfection efficiency. Therefore, each expression vector needs a fine tuning of transfection conditions ...

Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...