11月14日Nature MethodsNature Methods “A comprehensive human embryo reference tool using single-cell RNA-sequencing data”，研究人员采用scRNA-seq，对人类胚胎在不同发育阶段 ...

With a modified polymerase and optimized oligonucleotide chemistry, Helicos' single-molecule sequencer takes on RNA. RNA sequencing (RNA-seq) is actually a misnomer for the increasingly popular ...

引言人类胚胎发育的过程犹如一幅精妙绝伦的生命画卷，从一个微小的受精卵开始，短短数周内通过复杂而精准的细胞分裂与分化，演变成具有初步结构和功能的胚胎。这一过程不仅展现了生物学的奥妙，也蕴藏着生命起源的答案。尽管人类对自身的了解日益深入，胚胎发育的早期阶 ...

Conventional targeted RNA-seq methods often require labor-intensive pre-sequencing enrichment steps, which can compromise comprehensive transcriptome profiling and limit their broader applications.

It also provides useful information regarding this popular next-generation sequencing technique to aid researchers in selecting the best method to achieve optimal results for their RNA-Seq ...

7 Typical quality control methods used for this new class of therapies are plagued by high costs and long timelines, but according to these scientists, direct RNA-seq can be used to streamline the ...

Recent advancements in next-generation sequencing technologies have provided powerful tools for RNA modification detection, yet these methods often lack the sensitivity and specificity needed to ...

In this valuable study, the authors used an elegant genetic approach to delete EED at the post-neural crest induction stage. The usage of the single-cell RNA-seq analysis method is extremely suitable ...