RT–PCR is a variation of PCR, or polymerase chain reaction. The two techniques use the same process except that RT–PCR has an added step of reverse transcription of RNA to DNA, or RT, to allow for ...

SuperScript IV UniPrime One-Step RT-PCR System is designed to perform both cDNA synthesis and subsequent PCR amplification in a single tube. This master mix formulation combines the high processivity ...

Traditional PCR amplification is a qualitative assay ... The addition of a reverse transcription (RT) step to qPCR occurs when the target is RNA, such as when testing for the presence of SARS ...

The AAV viral vectors, as well as messenger RNAs derived from plasmid DNA templates, are crucial steps in the production ... maximum sensitivity. While real-time PCR is still the gold standard ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative ... control of possible carryover contamination. Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure ...