This protocol was adapted from “DNA transfection by electroporation” in Molecular Cloning: A Laboratory Manual (eds. Sambrook, J. & Russell, D.W.) 16.33–16.36 (Cold Spring Harbor Laboratory ...
Otherwise, it will be difficult to troubleshoot problems occurring with the technology, the transfection protocol and/or the biological assay. For the Nanog-GFP line shown in Figure 1, we use a ...
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