However, even when mutant mice are maintained correctly as a randomly bred colony or as a continually backcrossed congenic strain, there are potential confounds. Although studying a mutation on a ...

When hybrid animals mate with either of their parent species, they are known as backcrossed hybrids. If backcrossing ...

Transgenic plant lines containing independent transgenes and independent transgenic insertion events are backcrossed and evaluated at a number of different breeding locations. Within this process ...

Dozens of rodents have been generated that model various aspects of ALS, like motor impairment or degeneration of motor neurons. No model recapitulates the human disease perfectly. By organizing ...

To most people, all small rodents are virtually indistinguishable from each other, and as such, they are lumped together and considered to be mice of one kind or another. In Webster’s Third New ...

Mice express full-length protein ZBP1-L as well as an alternatively-spliced shorter isoform (ZBP1-S) containing only its two ...

Pathogenic activating point mutations in the LRRK2 kinase cause autosomal-dominant familial Parkinsońs disease (PD). In cultured cells, mutant LRRK2 causes a deficit in de novo cilia formation and ...

Sickness-induced sleep is a behavior conserved across species that promotes recovery from illness, yet the underlying mechanisms are poorly understood. Here, we show that interleukin-6-like cytokine ...

A colony should be established from N1 animals harboring the same sequence confirmed HDR allele. We recommend that N1 animals be backcrossed to wild-type animals. Intercrossing N1 animals is not ...

The APOE3 Targeted Replacement model is one line in a set of three APOE knock-in lines created by Nobuyo Maeda, Patrick Sullivan, and colleagues. APOE2 and APOE4 Targeted Replacement mice are also ...

Metabolic rewiring is essential for osteoclast differentiation and bone resorption. This study shows that glutaminolysis ...

Isolated Kmt2d-KO and littermate control thymic cells were centrifuged to pellet, resuspended in 300 µL of Protection Buffer (Monarch RNA Isolation Kit, #T2010S; New England Biolabs Inc. (NEB), ...