This protocol describes the first stages of Southern blotting: digestion of genomic DNA with restriction enzymes, separation of the resulting fragments by gel electrophoresis, and capillary ...
Northern blotting and hybridization are used to study gene expression by detecting RNA species of interest, and to identify alternate RNA splicing patterns. It is analogous to Southern blotting ...
Western blotting is a multi-step procedure that utilizes highly selective antibodies that bind to a specific protein of interest, allowing investigators to detect a specific protein in a complex ...
Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...