基因敲入（knockin）实验所面临的主要挑战之一就是供体模板的制备。 双链DNA(dsDNA)和单链DNA(ssDNA)都可以作为HDR修复机制的供体模板。不过，质粒或者PCR片段这样的dsDNA供体模板存在随机整合到基因组上的风险，在准确的基因编辑上的应用是非常有限的。而ssDNA ...

A new study in Nature identifies a long-sought cytoplasmic 'sensor' that is responsible, at least in part, for interferon responses induced by double-stranded DNA. How do cells detect non-self ...

The DeNovix dsDNA Fluorescence Quantification Assays and Fluorometers provide the greatest sensitivity and widest dynamic range available today. DeNovix offers three highly specific assay kits for ...

CRISPR/Cas-initiated HR in mouse embryos will be used to generate founder animals harboring inserted sequence targeted to the ROSA26 locus. The targeting will be done in embryos using microinjection ...