In contrast, off-target effects for siRNA are much more difficult to classify, as short stretches of sequence homology may exist among RNA molecules encoding structurally distinct classes of ...

They observed that introducing double-stranded RNA (dsRNA) into the worm led to the silencing of genes with complementary sequences. This discovery laid the foundation for understanding the mechanism ...

This methodology is achieved both quickly and easily in both animal and cell-line models. The success of this methodology depends on choosing the proper target sequence within the gene of interest and ...

In SNALPs, the backbone-modified siRNA is encapsulated within a closed shell of a cationic-zwitterionic lipid bilayer, furnished with an outer PEG shield (Figure 2A). The lipid bilayer contains a ...

Nanomedicine. 2014;9(1):105-120. Overall, HDL-based siRNA delivery possesses many attractive features, including ultra-small size, favorable surface properties (no PEGylation is required for ...