基因敲入所面临的主要难题是修复模板的制备和如何与Cas9-sgRNA核糖核蛋白复合物共导入至细胞。虽然单链DNA（ssDNA）修复模板展示出了优于双链DNA（dsDNA）模板的众多优势，但是其制备难度和制备费用都有很大的挑战性，因而限制了长单链DNA（long ssDNA）模板的 ...

Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...

Gel electrophoresis equipment, instruments, and supplies separate nucleic acids or proteins on the basis of size, electric charge, and other physical properties. Gel electrophoresis is useful in ...

The Chemidoc streamlines Western blotting in a five-step approach by combining traditional blotting techniques with innovative tools to allow improved band quantitation and quick assessment of ...

CAMBRIDGE, Mass.--(BUSINESS WIRE)--Kano Therapeutics, a biotechnology company pioneering single-stranded DNA (ssDNA) as a safe, efficient and flexible biomolecule for gene insertions, today ...

The quality of your sequencing template greatly affects the success of the sequencing reaction. Sequencing by capillary electrophoresis is highly sensitive to sample contamination. Potential ...

The E-Gel ® electrophoresis system is ideal for routine analysis of PCR products, restriction digests, and plasmid preparations. No gels to pour, no buffer to make, no staining/destaining ...