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蛋白相互作用Pull-Down实验的方法—艾普蒂生物
表达融合标签的蛋白质(如His、Flag标签等)的细胞裂解物或纯化的蛋白质。 目标蛋白质的细胞裂解物或纯化的蛋白质。 裂解缓冲液(例如含有Tris-HCl、NaC 蛋白酶抑制剂等) 洗涤缓冲液(裂解缓冲液中增加适量的NaCl以减少非特异性结合) 洗脱缓冲液(针对特定 ...
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