After 8 hours of incubation, the agarose beads were washed with lysis buffer. For protein purification, the immunoprecipitated samples were eluted with 3xFLAG peptide or glycine (pH 2.5) neutralized ...

The cell lysates were pre-cleared and then incubated with pre-washed anti-Flag M2 agarose beads (Yeasen, #20584ES08) overnight at 4°C. Beads were washed three times with PBS. Protein samples were then ...