and DNA methylation or gene imprinting all influence cloning efficiency, I believe another factor is critical in determining success. It has been argued that the variable quality of oocytes ...
To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA (for ... CGFP constructs (steps 1 and 4 ...
Nov. 7, 2024 — Researchers have used ancient DNA to challenge long-held interpretations of the people of Pompeii. Contrary to physical appearances, the DNA evidence revealed unexpected ...
Nov. 18, 2024 — People with chronic liver disease have dramatic changes to the types and functions of bacteria in the gut and mouth, a new study has ...
C) Any cDNA template can be converted to Gateway system using PCR in GBU. (ideal cDNA length 1-2,5 kb) Step 2: The cDNA is cloned to pAAV-Gateway using Gateway cloning. Step 3: Helvi-AAV produces the ...
Differences in the ribosomal densities of the two daughters produced by a mother Escherichia coli could explain the asymmetry of aging and growth rate in previously reported such daughter pairs.