1. Preparation of a cell extract: To extract DNA from a tissue/cells of interest, in this experiment the heart muscle of rats, the cells have to be separated and the cell membranes have to be ...

This protocol was adapted from “Purification of RNA from Cells and Tissues by Acid Phenol-Guanidinium Thiocyanate-Chloroform Extraction,” in Molecular Cloning: A Laboratory Manual (eds.