基因敲入所面临的主要难题是修复模板的制备和如何与Cas9-sgRNA核糖核蛋白复合物共导入至细胞。虽然单链DNA（ssDNA）修复模板展示出了优于双链DNA（dsDNA）模板的众多优势，但是其制备难度和制备费用都有很大的挑战性，因而限制了长单链DNA（long ssDNA）模板的 ...

基因敲入（knockin）实验所面临的主要挑战之一就是供体模板的制备。 双链DNA(dsDNA)和单链DNA(ssDNA)都可以作为HDR修复机制的供体模板。不过，质粒或者PCR片段这样的dsDNA供体模板存在随机整合到基因组上的风险，在准确的基因编辑上的应用是非常有限的。而ssDNA ...

Protocol is a format for step-by-step descriptions of procedures that users can take to the lab and immediately apply in their own research. All of our protocols have been proven to work already ...

The Kyoto Protocol, the first international treaty to set legally binding targets to cut greenhouse gas emissions, was adoped 25 years ago, on 11 December 1997, in Kyoto, Japan. The agreement ...

However, now that a topical gel for ED, Eroxon, is available over-the-counter without a prescription, a big question is whether discussions about ED will become more, well, topical, natural and ...

We direct and coordinate the international activities conducted in these situations. The Protocol Officer ensures that departmental activities are organized smoothly, including coordination of pick-up ...

Microsoft has officially deprecated the Point-to-Point Tunneling Protocol (PPTP) and Layer 2 Tunneling Protocol (L2TP) in future versions of Windows Server, recommending admins switch to different ...

Whether it’s from acne, surgery or another wound-inducing experience, skin scarring is almost as inevitable as it is permanent. For most scars that create textural changes to your skin, it ...

Skin experts are warning a chemical found in gel, gel polish and acrylic nails can cause an allergic reaction. Methacrylate chemicals can cause a severe, itchy rash anywhere on the body ...

Both Lo and Gerstein swear by this inky formula for a multitude of eyeliner looks. “I love Bobbi Brown’s gel eyeliner because it glides on smoothly—whether you want a subtle line or a bold ...

From acrylics to dip powder nails, there have been many variations of artificial nails we've seen become trendy, but there's ...