Simple stains with fluorochrome dyes—acridine orange, for example—are inadequate for diagnostic use. Two methods which have hitherto been used for the demonstration of polysaccharides proved ...

Spectral flow cytometry collects the full emission spectrum of a fluorochrome, enabling multicolor panels with more parameters than conventional flow cytometry. As scientists discover the subtle ...

However, choosing optimal antibody-fluorochrome combinations is a complex process. Here we provide some simple guidelines for the selection of reagent panels for multicolor flow cytometry.