Most work on ubiquitination site determination has been done with in-gel digestion using iodoacetamide at relatively high concentrations. Previously reported sites, especially those discovered in ...

Protein separation using 1D SDS-PAGE. Proteolytic Digestion: In-solution or in-gel digestion performed using our DigestPro automated digestion unit to minimise sample handling. Peptide fractionation ...

This protocol describes the first stages of Southern blotting: digestion of genomic DNA with restriction enzymes, separation of the resulting fragments by gel electrophoresis, and capillary ...