CA); 2.5 μl of ligation reaction were subjected to a second round of PCR amplification in 100 μl reaction volume under the same conditions, except that the M13-20 primer was used instead of the ...

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Furthermore, the presence of PARP1 condensates significantly promotes DNA ligation upon PARylation. These findings provide insight into how PARP1 condensation and PARylation regulate the assembly and ...

(D) Quantification of AICD-Flag peptides from enzyme reactions of recombinant APP substrate C100-Flag with WT versus FAD-mutant proteases by MALDI-TOF MS. Standard curves were used to quantify AICD ...

SPAAC is a kind of click reaction without any metal and enzyme between azides and cyclooctyl alkynes, which has high selectivity and biological orthogonality. It is suitable for the ligation of ...

Allergies are an immune system reaction to substances called allergens. The cause a range of symptoms from a stuffy nose to itchy eyes to difficulty breathing. How long allergies last varies based on ...

A systemic reaction occurs when inflammation spreads from a localized area of one organ (like the skin) to other organ systems in the body. It is different from a localized reaction, which is a ...

Institute for Advanced Chemistry of Catalonia (IQAC), Spanish National Research Council (CSIC), C/ Jordi Girona 18-26, 08034 Barcelona, Spain Department of Inorganic and Organic Chemistry, Section of ...

The CyBio FeliX, a benchtop instrument for automated liquid handling tasks, was used to automate the HaloPlex Target ...

Here, we used deep full-length single-cell RNA-sequencing to distinguish post-mitotic cone and rod developmental states and cone-specific features that contribute to retinoblastomagenesis. The ...

The Raptors are still searching for their first road win after losing to the Cavs.