Researchers developed novel genetic tools to trace, ablate, and manipulate p16^Ink4a+^ senescent cells, revealing their ...

In this important study, the authors used a zebrafish model and scRNAseq analysis to show that a subset of keratinocytes within melanoma microenvironment highly up-regulate Twist and undergo ...

This useful study by Gao et al identifies Hspa2 as a heterogeneous transcript in the early embryo and proposes a plausible mechanism showing interactions with Carm1. The authors propose that ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Wnt signaling is an important target for ...

其他：SDS-PAGE试剂，PVDF膜，化学发光试剂（ECL），转染试剂。 细胞培养：将HEK293或HeLa细胞培养至约50-70%融合度。 细胞转染： 使用Lipofectamine 3000或其他合适的转染试剂，将SGTA过表达质粒或SGTA siRNA转染到细胞中。 阴性对照：对于过表达实验，使用空载体质粒转染 ...

Transduced HUVECs were selected for positive genetic inactivation by puromycin selection for 2 weeks. Transfections of endothelial cells were performed using a Lipofectamine 3000 kit (Thermo Fisher ...