UV-Vis-NIR reflectance spectra (250 to 1000 nm) were obtained using a 10× UV-absorbing glass objective lens, with a minimum of five replicates per sample ... and examined the imaging under white or UV ...
image quality is affected by both scattering in the biological medium and by an index-of-refraction mismatch between the tissue and the water/oil on which the microscope objective is optimized.