同源重组修复的关键基因发生突变会导致小鼠胚胎致死。在同源重组修复过程中，复制蛋白A（RPA）能够迅速识别末端切除产生的3′端ssDNA并与之结合。随后，重组酶Rad51需要及时替换RPA与ssDNA结合形成Rad51核蛋白纤丝，从而进行同源模板搜索、链入侵及后续修复 ...

In contrast to the previously characterized type I systems known for uncontrolled cleavage and preferential targeting of dsDNA, this type I-F variant has been demonstrated to precisely cleave both ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. The evolving genetic landscape of inherited ...

By mixing ddNTPs with regular nucleotides and analysing the results through gel electrophoresis ... used for sequencing were single-stranded DNA (ssDNA) typically derived from fragments cloned ...

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The E-Gel ® electrophoresis system is ideal for routine analysis of PCR products, restriction digests, and plasmid preparations. No gels to pour, no buffer to make, no staining/destaining ...

Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...

1 Department of Biochemistry, Institute of Medical Biology, University of Tromsø, N-9037 Tromsø, Norway 2 Department of Rheumatology, Institute of Clinical Medicine, University of Tromsø, N-9037 ...