In this study a cationic hydrogel (quaternized dimethyl aminoethyl methacrylate, QDM) was reversibly bonded to bovine aorta and other tissues, with said tissues functioning as the anionic element.

Cells were collected by centrifugation and resuspended in Ni-NTA Bind Buffer containing 4-(2-aminoethyl)benzenesulfonylfluoride·HCl (AEBSF hydrochloride), benzamidine hydrochloride and Lysonase.