Engineering the replication of target DNA through cloning, or changing its genetic code through mutations, are detail-oriented processes whose foibles can spell disaster. Caitlin Smith looks at ...

In step 2, the gene fragment is integrated into a circular piece of DNA, called a plasmid, which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid ...

Cloning is a biotechnological process that involves creating genetically identical copies of an organism or cell. In essence, a clone is an exact genetic replica of the original organism or cell, ...

Figure 2 shows the configuration of a sample template DNA oligonucleotide derived from the RNA oligonucleotide design. Synthesize or order the template oligonucleotide designed in steps 1 and 2 ...