Each cycle of PCR has three main steps, as described in the following ... Once PCR cycling is complete, the copied DNA molecules can be used for cloning, sequencing, mapping mutations, or studying ...

Cloning is a biotechnological process that involves creating genetically identical copies of an organism or cell. In essence, a clone is an exact genetic replica of the original organism or cell, ...

In step 2, the gene fragment is integrated into a circular piece of DNA, called a plasmid, which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid ...