This protocol describes a single-step technique for the purification of RNA. Cells are homogenized in guanidinium thiocyanate and the RNA is purified from the lysate by extraction with phenol ...

One method involves in situ crosslinking followed by the purification ... a specific protein 5. However, introduction of DNA breaks is likely to cause major changes in chromatin structure and ...

2. Purification of DNA from cell extract In addition to DNA the cell extract will contain significant quantities of protein and RNA. A variety of procedures can be used to remove these contaminants, ...