We performed specific staining for superoxide anions using MitoSOX and we observed a lower magnitude of differences than for DCFDA-AM, suggesting that other ROS species are involved in the process.

Taylor Swift had one big takeaway from Katy Perry‘s Video Vanguard Award speech at the 2024 MTV Video Music Awards. Swift, 34, gave Perry, 39, a standing ovation as the “Roar” singer ...

For selected experiments, cells were incubated with 1 nM MitoTracker Deep Red (Invitrogen, M22426), 1 μM MitoSOX Red (Invitrogen, M36008), for 30′ min or 1: ... for raw data unmixing and downstream ...

The single cells in PBS were analysed with a BD LSR Fortessa x-20 FACS Analyzer and FlowJo (v10.6.1). Two days prior to starting differentiation, cells were dissociated using Accutase and seeded as ...

Aging affects iron homeostasis, as evidenced by tissue iron loading and anemia in the elderly. Iron needs in mammals are met primarily by iron recycling from senescent red blood cells (RBCs), a task ...

After extensive washes, the cells were resuspended and analyzed with BD LSRFortassa (BD Biosciences) and FlowJo v10 software (FlowJo). HEK 293T cells without transfection were also stained as a ...