Departments of Pharmacology, UT Southwestern Medical Center, Dallas, USA Departments of Peter O’Donnell Jr. Brain Institute and Psychiatry, UT Southwestern Medical Center, Dallas, USA Department of ...
We purified GST fusion proteins on glutathione agarose beads (20 μl) in buffer H (120 mM KOAc, 20 mM Tris-OAc, pH 7.9, 1 mM EDTA, 20% glycerol). Equal amounts of GST fusion proteins were used in ...
In summary, these BLI data demonstrate that B. subtilis MinD is capable of binding membrane in both monomeric and dimeric form, while the presence of ATP in the binding pocket appears to affect both ...