1 Department of Biochemistry, Institute of Medical Biology, University of Tromsø, N-9037 Tromsø, Norway 2 Department of Rheumatology, Institute of Clinical Medicine, University of Tromsø, N-9037 ...
2015; Shibuya et al., 2014; Zhang et al., 2017). Although telomeres have ancient evolutionarily conserved roles and conserved DNA sequences, the telomeric double-strand DNA (dsDNA)-binding proteins ...
The DeNovix dsDNA Fluorescence Quantification Assays and Fluorometers provide the greatest sensitivity and widest dynamic range available today. DeNovix offers three highly specific assay kits for ...
There were no differences in sex distribution or duration of follow up. A higher proportion of anti-dsDNA, anti-RNP, anti-Sm, and lupus anticoagulant (LA) was seen in the group with nephritis (p=0.002 ...
CRISPR/Cas-initiated HR in mouse embryos will be used to generate founder animals harboring inserted sequence targeted to the ROSA26 locus. The targeting will be done in embryos using microinjection ...
To favor HDR, many approaches interfere with the repair machinery or manipulate Cas9 itself. Using Medaka we show that the modification of 5’ ends of long dsDNA donors strongly enhances HDR, favors ...
NRR at 6 months (NRR6) was the strongest negative predictor of CRR at 12 months. Baseline anti-dsDNA positivity inversely predicted NRR6 (OR=0.32,95% CI=0.10 to 0.98, p=0.049), while anti-SSA/Ro60 ...
ELISA for ANAs and anti-double-stranded DNA (dsDNA) antibodies The quantity of ANA and dsDNA ... horseradish peroxidase (HRP)-conjugated secondary antibody, followed by DAB immunostaining and ...
基因敲入（knockin）实验所面临的主要挑战之一就是供体模板的制备。 双链DNA(dsDNA)和单链DNA(ssDNA)都可以作为HDR修复机制的供体模板。不过，质粒或者PCR片段这样的dsDNA供体模板存在随机整合到 ...
虽然单链DNA（ssDNA）修复模板展示出了优于双链DNA（dsDNA）模板的众多优势，但是其制备难度和制备费用都有很大的挑战性，因而限制了长单链DNA（long ssDNA）模板的应用。Guide-it Long ssDNA Strandase ...
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