Stat3 Antibody - Cell Signaling Technology
Polyclonal Antibody for studying STAT3. Validated for ChIP, IHC, IP, WB. Highly specific and rigorously validated in-house, Stat3 Antibody (CST #9132) is ready to ship.
STAT3 (STAT3)靶点介绍及实验小贴士| Abcam中文官网
stat3 (stat3)靶点介绍助您了解其蛋白功能，蛋白定位及异构体和翻译后修饰。同时列举了实用的wb实验小贴士，助您实验成功。
Quantification of total and phosphorylated STAT3 by calibrated western …
2023年9月15日 · To determine the efficiencies of the IPs, the amounts of STAT3 and (p)Y STAT3 in aliquots of the lysate before (Pre) and after (Post) IP are monitored in a semi-quantitative western blot. In the following, STAT3 will serve as example for the quantification of intracellular signaling proteins.
We present isolation of total and phosphorylated STAT3 from cell lysates by immunoprecipitation, followed by SDS-PAGE and western blot together with known amounts of a calibrator protein that shares an epitope with the precipitated proteins.
Anti-STAT3 Antibodies | Invitrogen - Thermo Fisher Scientific
Antibodies that detect STAT3 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Flow Cytometry. These antibodies target STAT3 in Human, Mouse, Rat, Non-human primate and Rabbit samples.
Visualization and quantification of dynamic STAT3 ... - Nature
2018年2月5日 · We were able to differentiate between endogenous STAT3 (~88 kDa) and exogenous Fluoppi (PB1 and mAG1) tagged STAT3 (~130 kDa) by western blotting because of their different molecular...
Targeting polarized phenotype of microglia via IL6/JAK2/STAT3
2022年2月23日 · Western blot. Western blot was performed as previously published. Cells were lysed using radioimmunoprecipitation assay (RIPA; Beyotime, Shanghai, China) buffer.
The antioxidative effect of STAT3 involved in cellular vulnerability …
2024年12月4日 · To investigate the protective role of STAT3 against isoflurane-induced cytotoxicity, U251 cells were transiently transfected with a wild-type STAT3 gene-containing vector, STAT3-pcDNA3. Western blot analysis confirmed that the cells transfected with STAT3-pcDNA3 expressed high levels of STAT3 protein (Fig. 3A; n = 4, P < 0.001) as well as its ...
Unraveling the complexity of STAT3 in cancer: molecular …
2024年1月20日 · We summarize both the positive and negative modulators of STAT3 together with the cancer hallmarks involving activities regulated by STAT3 and highlight its extremely sophisticated regulation on immunosuppression in tumor microenvironment and …
Phospho-Stat3 (Tyr705) Antibody | Cell Signaling Technology
Phospho-Stat3 (Tyr705) Antibody detects endogenous levels of Stat3 only when phosphorylated at Tyr705. The antibody does not cross-react with other Stat proteins when phosphorylated on the corresponding tyrosine residue, but has been shown to cross-react with Phospho-EGFR.
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