A cross-chiral RNA polymerase ribozyme - Nature
2014年10月29日 · The ribozyme is thought to interact with its substrates via tertiary contacts rather than Watson–Crick base pairing. This unexpected finding will add a new dimension to thoughts on how life ...
Prebiotic chiral transfer from self-aminoacylating ribozymes
2024年9月12日 · Using a model system of self-aminoacylating D-ribozymes and epimerizable activated amino acid analogs, we test the chiral selectivity of 15 ribozymes derived from an exhaustive search of sequence...
Mirror-image T7 transcription of chirally inverted ribosomal and ...
2022年10月27日 · Here, we chemically synthesized a 100-kilodalton mirror-image T7 RNA polymerase, which enabled efficient and faithful transcription of the full-length mirror-image 5 S, 16 S, and 23 S ribosomal RNAs from enzymatically assembled long mirror-image genes.
Ribozyme crosses chirality borders - Nature
2015年1月7日 · Now, Sczepanski and Joyce show that polymerization of both D - and L -RNA may have emerged together, thanks to ribozymes capable of cross-chiral RNA polymerization. By using in vitro evolution,...
The model structure of the hammerhead ribozyme formed by …
2021年1月1日 · The model of mixed chirality between L-hammerhead ribozyme with D-RNA substrate is based on nucleotide sequence complementarity. Two short heterochiral helixes (helices 1 and 3) are formed between flanking parts of L-ribozyme with the substrate, but helix 2 of the catalytic L-RNA is always homochiral and stable.
Cross-chiral exponential amplification of an RNA enzyme
2024年10月22日 · An RNA ligase ribozyme that catalyzes the joining of RNA molecules of the opposite chiral handedness was optimized for the ability to synthesize its own enantiomer from two component fragments. The mirror-image D- and L-ligases operate in concert to provide a system for cross-chiral replication, whe …
Cross-chiral-Ribozyme-Hold-Clues - Chemical & Engineering News
2014年11月4日 · Researchers have developed the first cross-chiral biocatalyst—a right-handed (D) RNA enzyme that catalyzes the linkage of left-handed (L) RNA nucleotides into L-RNA chains. No other RNA or protein enzymes of one chirality are known to use substrates of the opposite chirality to produce biopolymers.
Prebiotic chiral transfer from self-aminoacylating ribozymes may …
2024年9月24日 · Using a model system of self-aminoacylating D-ribozymes and epimerizable activated amino acid analogs, we test the chiral selectivity of 15 ribozymes derived from an exhaustive search of sequence space. All of the ribozymes exhibit detectable selectivity, and a substantial fraction react preferentially to produce the D-enantiomer of the product.
A Cross-chiral RNA Polymerase Ribozyme - PMC - National …
2014年11月11日 · Cross-chiral assembly of long RNAs a, Assembly of 50mer and 49mer D-RNAs on complementary D-RNA templates through multiple ligation and polymerization events, catalyzed by the L-RNA enzyme. The reaction mixtures were sampled at 0, 1, 2, and 3 d and the 5′-labeled products were analyzed by polyacrylamide gel electrophoresis in comparison to ...
Chirality - SpringerLink
2023年1月1日 · The term chirality, from the Greek word cheir for “hand,” refers to the property of “handedness” possessed by some molecules and macroscopic objects. A molecule or object is chiral if it is not superimposable on its mirror image, as is the case for left and right hands.