Microbial DNA in Human Body Can Be Used to Identify Individuals
2015年5月13日 · on May 11, suggests that it might be possible to identify a participant in an anonymous study of the body’s microbial denizens—its microbiome—and to reveal details about that person’s health,...
Microbial genetics - Wikipedia
Microbial genetics is a subject area within microbiology and genetic engineering. Microbial genetics studies microorganisms for different purposes. The microorganisms that are observed are bacteria and archaea. Some fungi and protozoa are also subjects used to study in this field.
An Improved Method for High Quality Metagenomics DNA
2016年5月31日 · Efficient extraction of high-quality, high molecular weight (HMW) community genomic DNA from limited amount of human origin or environmental samples carrying diverse microbial species is the...
11: Mechanisms of Microbial Genetics - Biology LibreTexts
2024年4月21日 · All cells use DNA for information storage, share the same genetic code, and use similar mechanisms to replicate and express it. Although many aspects of genetics are universally shared, variations do exist among contemporary genetic systems.
Genetics - Medical Microbiology - NCBI Bookshelf
Recombinant DNA and gene cloning are essential tools for research in molecular microbiology and medicine. They have many medical applications, including development of new vaccines, biologics, diagnostic tests, and therapeutic methods. Expression of genes in microbes is often regulated by intracellular or environmental conditions.
Evaluation of Methods for the Extraction and Purification of DNA …
Six commonly used DNA extraction methods that employed different mechanisms for cell lysis and DNA purification were statistically evaluated according to the following criteria: DNA yield, DNA shearing, representation of microbial diversity, and reproducibility.
DNA extraction of microbial DNA directly from infected tissue: …
2020年2月19日 · Performing shotgun metagenomic sequencing on bacterial DNA extracted directly from the infected tissue may improve time to diagnosis and targeted treatment considerably. However, infected tissue...
Principles and Concepts of DNA Replication in Bacteria, …
The core machineries that copy DNA are conserved in all three domains of life: bacteria, archaea, and eukaryotes. This article outlines the general nature of the DNA replication machinery, but also points out important and key differences.
Bacterial DNA - an overview | ScienceDirect Topics
Bacterial DNA is organised in a compact nucleoid body that is tightly associated with the coupled transcription and translation of mRNAs. This structure contains abundant DNA-binding proteins which perform both structural and regulatory roles, and, in Escherichia coli, serve to buffer and organise pervasive DNA superhelicity.
Analytical and clinical validation of a microbial cell-free DNA ...
2019年2月11日 · Here we describe the analytical and clinical validation of a next-generation sequencing test that identifies and quantifies microbial cell-free DNA in plasma from 1,250 clinically relevant...
8.5: Structure and Organization of DNA in Bacteria
Sexual reproduction allows compatible genders (think male and female) to share genes, a strategy that increases species diversity. It turns out that bacteria and other single celled organisms can also share genes… and spread diversity.
11.2: DNA Replication - Biology LibreTexts
2024年4月21日 · For bacterial DNA replication to begin, the supercoiled chromosome is relaxed by topoisomerase II, also called DNA gyrase. An enzyme called helicase then separates the DNA strands by breaking the hydrogen bonds between the nitrogenous base pairs.
Bacterial DNA - an overview | ScienceDirect Topics
Bacterial DNA is the genetic material of bacteria that is organized into a superhelical structure called the nucleoid, which is not enclosed within a membrane-bound nucleus like in eukaryotes. It typically ranges in size from 0.7 to 10 megabases and is essential for replication, transcription, and repair processes in bacteria.
Bacterial DNA – the role of plasmids — Science Learning Hub
The DNA of most bacteria is contained in a single circular molecule, called the bacterial chromosome. The chromosome, along with several proteins and RNA molecules, forms an irregularly shaped structure called the nucleoid.
Sequencing-based analysis of microbiomes - Nature
2024年6月25日 · In this Review, Pinto and Bhatt provide an overview of DNA-sequencing and RNA-sequencing approaches that can be used to study the composition, structure, and function of microbiomes and discuss...
Bacterial Artificial Chromosome (BAC) - National Human …
2 天之前 · A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs. The BACs, with their inserted DNA, are ...
Microbial DNA barcoding - Wikipedia
Microbial DNA barcoding is the use of DNA metabarcoding to characterize a mixture of microorganisms. ... DNA barcoding of cyanobacteria can be applied in various ecological, evolutionary and taxonomical studies. Some examples include assessment of cyanobacterial diversity and community structure, ...
Microbial metabolites damage DNA | Science - AAAS
2022年10月27日 · By screening 122 bacterial isolates from IBD patients in a cell-free DNA assay, the authors identify 18 strains with different types of DNA-damaging activity, including suspected DNA alkylation and DNase-like digestion of a linearized plasmid.
Bacterial DNA excision repair pathways - Nature
2022年2月24日 · To maintain genome integrity and ensure cell survival, bacteria have evolved several DNA repair pathways to correct different types of DNA damage and non-canonical bases, including strand...
7: Microbial Genetics - Biology LibreTexts
A plasmid is a small DNA molecule that is physically separate from, and can replicate independently of, chromosomal DNA within a cell. Plasmids are commonly used to multiply (make many copies of) or express particular genes.
New research maps microbial life in U.S. rivers - Conservation news
3 天之前 · With the help of the public, scientists mapped microbial life across 90% of U.S. watersheds by analyzing genetic data from approximately 100 rivers, creating the first comprehensive catalog of U.S ...
The bacterial epigenome | Nature Reviews Microbiology
2019年11月14日 · In bacteria, DNA methylation has roles in genome defence, chromosome replication and segregation, nucleoid organization, cell cycle control, DNA repair and regulation of transcription.
Host DNA depletion on frozen human respiratory samples enables …
4 天之前 · To determine the amount of microbial DNA in intact microbial cells versus extracellular DNA, 1 mL of each liquid culture was centrifuged at 10,000 × g for 3 min. The supernatant was syringe ...