Improving cell-adhesive properties of recombinant Bombyx mori …
Silk of Bombyx mori can be used as various biomaterials. Especially, it is useful as a protein for coating the surface of cell culture plates since the silk possesses a biocompatibility to the cultured cells. However, the cell-adhesive ability is weaker than collagen or fibronectin, which are used f …
High yield exogenous protein HPL production in the Bombyx mori …
2015年9月15日 · Yanagisawa, S. et al. Improving cell-adhesive properties of recombinant Bombyx mori silk by incorporation of collagen or fibronectin derived peptides produced by transgenic silkworms ...
Injectable Bombyx mori (B. mori) silk fibroin/MXene conductive …
2024年3月14日 · Bombyx mori (B. mori) silk fibroin (SF), one of the natural biomaterials, has been considered a particularly promising candidate by having exceptional mechanical properties, excellent biocompatibility, and controllable biodegradability .
Materials fabrication from Bombyx mori silk fibroin - Nature
2011年9月22日 · In this protocol, we include methods to extract silk from B. mori cocoons to fabricate hydrogels, tubes, sponges, composites, fibers, microspheres and thin films. These materials can be used...
Generation of hybrid transgenic silkworms that express Bombyx mori ...
2006年11月1日 · In this study we aimed at generating hybrid transgenic silkworms whose PSGs are capable of producing mini-collagens and enough P4H for their prolyl 4-hydroxylation. Isolated PSGs were bombarded with fibroin L-chain gene promoter-driven vectors containing Bombyx mori P4Hα (BmP4Hα) cDNAs and were transplanted into the hemolymphatic cavity. The ...
Synthesis of prolyl 4-hydroxylase α subunit and type IV collagen …
2005年4月1日 · The present study shows that hemocytic granular cells synthesize and secrete type IV collagen (ColIV) in the silkworm Bombyx mori ( B. mori) and suggests that these cells play roles in the formation of basement membrane, the encapsulation of foreign bodies, and the metamorphic remodeling of the gut.
Synthesis of prolyl 4-hydroxylase alpha subunit and type IV collagen …
The present study shows that hemocytic granular cells synthesize and secrete type IV collagen (ColIV) in the silkworm Bombyx mori (B. mori) and suggests that these cells play roles in the formation of basement membrane, the encapsulation of foreign bodies, and the metamorphic remodeling of the gut.
Transgenic silkworms produce recombinant human type III
2002年12月16日 · Thus, there is a need for alternative sources of collagen to produce large quantities. The domestic silkworm, Bombyx mori, synthesizes vast amounts of silk protein in its silk glands and...
The advances and perspectives of recombinant protein production …
2014年8月12日 · The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be widely used in the biological ...
Structure characteristics and expression profiles of Bombyx mori …
2010年11月10日 · Here, we introduced a way to quickly and effectively do research on Bmlet-2 (BmColIVα1) gene from Bombyx mori, a central model for Lepidoptera. BmColIVα 1 gene is 6.583 kbp in full length, containing four exons, and its cDNA is 3.847 kbp, which contains an ORF for a protein of 819 amino acids with a molecular weight of 82.1 kDa and an ...