如何长效抑制miRNA ——miRNA sponges 必须了解下 - 知乎
网页microRNA (miRNA) 广泛参与调节动物的发育过程、生理反应和病理，通过与编码基因mRNA的互作调控后者的表达，再加近几年参与非编码基因 (lncRNA、circRNA)-miRNA-mRNA的ceRNA网络，又催生了miRNA的研究热潮。. 分子的机制研究少不了对其在细胞或者动物水平进行过表达或者 ...
MicroRNA sponges: Progress and possibilities - PMC
网页The microRNA (miRNA) “sponge” method was introduced three years ago as a means to create continuous miRNA loss of function in cell lines and transgenic organisms. Sponge RNAs contain complementary binding sites to a miRNA of interest, and are produced from transgenes within cells.
miRNA海绵—长效抑制miRNA-丁香实验 - biomart.cn
网页2010年3月15日 · miR-SP能鉴定组织特异性的miRNA功能丧失表型，确定效应器的空间调节，并了解miRNA与其他基因之间的相互作用。 研究人员发现这种构建体与Gal4的组合能表达出足够的miRNA沉默物，在完整的果蝇中实现miRNA的时空抑制。
驾驭基因表达调控（6）关于 miRNA 海绵（miRNA sponge）你是否有很多问号？_Sponge
网页2020年5月16日 · 为此，麻省理工大学研究人员开发了 miRNA sponge 技术，用于长效抑制多种具有相同功能的 miRNA。. 该技术将串联重复（6x或8x）miRNA 结合域克隆至由 CMV 启动子调控的载体上转染宿主后，miRNA sponge 以 mRNA 的形式转录至胞质中，诱导胞质中具有相似结合域的 miRNA 结合 ...
miRNA sponges: soaking up miRNAs for regulation of gene …
网页Endogenous miRNA sponges, also termed competing endogenous RNAs (ceRNAs), act to buffer the activity of miRNAs on physiologically relevant targets. This class of sponges includes endogenously transcribed pseudogenes, long noncoding RNAs, and recently discovered circular RNAs and may act in large complex networks in …
MicroRNA sponges: competitive inhibitors of small RNAs in
网页2007年8月12日 · They specifically inhibit microRNAs with a complementary heptameric seed, such that a single sponge can be used to block an entire microRNA seed family. RNA polymerase II promoter (Pol II)-driven...
miRNAsong: a web-based tool for generation and testing of miRNA sponge …
网页2016年11月18日 · MicroRNA (miRNA) sponges are RNA transcripts containing multiple high-affinity binding sites that associate with and sequester specific miRNAs to prevent them from interacting with their...
Natural RNA circles function as efficient microRNA sponges
网页2013年3月21日 · Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner.
MicroRNA sponges: Progress and possibilities - CSHL P
网页The microRNA (miRNA) “sponge” method was introduced three years ago as a means to create continuous miRNA loss of function in cell lines and transgenic organisms. Sponge RNAs contain complementary binding sites to a miRNA of interest, and are produced from transgenes within cells.
Definition and identification of small RNA sponges: Focus on miRNA …
网页2017年3月15日 · The workflow proposes three ways to identify the miRNA sponge or the sequestered miRNA on the sponge. Strategies are based on purification from a bait (as 3′-UTR fused with MS2 motif or biotinylated miRNA) or a …