Molecular cloning and characterization of the pig MHC class
Major histocompatibility complex (MHC) class Ⅰ-related protein 1 (MR1), the most highly conserved MHC class Ⅰ molecule among mammals, is the restricting molecule for mucosal-associated invariant T (MAIT) cells. MAIT cells, a novel subset of T cells, play important roles in modulating the immune resp …
Anti Pig SLA Class I Antibody, clone JM1E3 - Bio-Rad
Mouse anti Pig SLA Class I antibody, clone JM1E3 recognizes a monomorphic epitope expressed by porcine MHC class I molecules (SLA - 1). SLA - 1 is expressed by all nucleated porcine cells, but not on erythrocytes. This antibody has also been shown to cross-react with human MHC Class I, including HLA-E. (Galiani et al. 2002)
Molecular cloning and characterization of the pig MHC class Ⅰ …
2019年7月1日 — Major histocompatibility complex (MHC) class Ⅰ-related protein 1 (MR1), the most highly conserved MHC class Ⅰ molecule among mammals, is the restricting molecule for mucosal-associated invariant T (MAIT) cells.
The genomic sequence and analysis of the swine major …
2006年7月1日 — We describe the generation and analysis of an integrated sequence map of a 2.4-Mb region of pig chromosome 7, comprising the classical class I region, the extended and classical class II regions, and the class III region of the major histocompatibility complex (MHC), also known as swine leukocyte antigen (SLA) complex.
Fulltext – Journal of Animal Breeding and Genomics - JABG
The genetic diversity of the major histocompatibility complex (MHC) molecules of pigs has not been thoroughly characterized. In this study, we successfully typed SLA-2, a pig MHC class I gene, from 10 pigs of five different breeds using a high-resolution typing method.
Creating Class I MHC Null Pigs Using gRNA and the Cas9 …
2014年12月1日 — We report the creation and characterization of class I MHC knockout pigs using the Cas9 nuclease and gRNAs. Pig fetal fibroblasts were genetically engineered using Cas9 and gRNAs, and class I MHC negative cells were then used as nuclear donors for somatic cell nuclear transfer. We produced 3 piglets devoid of all cell surface class I proteins.
The Phylogenetic History of the MHC Class I Gene Families in Pig ...
More than 990 kb of the 1200 kb in the SLA class I region of the pig major histocompatibility complex (MHC) have been sequenced. The present study was designed to establish the evolution of this region which was best understood by distinguishing three periods.
Regulation of porcine classical and nonclassical MHC class I
2007年3月10日 — Sequencing of the MHC class I region of the H01 (Hp-1.1) haplotype pig has provided a more detailed understanding of gene content, intron, and exon organization and promoter structure, allowing predictions of expression to be made (Renard et al. 2001, 2006).
Porcine major histocompatibility complex (MHC) class I molecules …
2011年7月8日 — We have recently developed efficient methods to generate recombinant human MHC-I (also known as human leukocyte antigen class I, HLA-I) molecules, accompanying peptide-binding assays and predictors, and HLA tetramers for specific CTL staining and manipulation. This has enabled a complete mapping of all HLA-I …
Novel SLA class I alleles of Chinese pig strains and their ... - Nature
2003年8月1日 — This study further reveals that the homologies of MHC class I genes in their primary structures and the deduced amino acids between Chinese pigs (SLA) and human (HLA-A*0201) are better than those...